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Diagnosis of Vibrio parahaemolyticus

Tests and diagnosis discussion for Vibrio parahaemolyticus:

FDA Bad Bug Book (Excerpt)

Food Analysis: FDA's Bacteriological Analytical Manual Vibro parahaemolyticus Methods used to isolate this organism from foods are similar to those used with diarrheic stools. Because many food isolates are nonpathogenic, pathogenicity of all food isolates must be demonstrated. Although the demonstration of the Kanagawa hemolysin was long considered indicative of pathogenicity, this is now uncertain. (Source: FDA Bad Bug Book)

FDA Bad Bug Book (Excerpt)

Diagnosis of Human Illness: Diagnosis of gastroenteritis caused by this organism is made by culturing the organism from the diarrheic stools of an individual. (Source: FDA Bad Bug Book)

Vibrio parahaemolyticus (General): DBMD (Excerpt)

Vibrio organisms can be isolated from cultures of stool, wound, or blood. For isolation from stool, use of a selective medium that has thiosulfate, citrate, bile salts, and sucrose (TCBS agar) is recommended. If there is clinical suspicion for infection with this organism, the microbiology laboratory should be notified so that they will perform cultures using this medium. A physician should suspect V. parahaemolyticus infection if a patient has watery diarrhea and has eaten raw or undercooked seafood, especially oysters, or when a wound infection occurs after exposure to seawater. (Source: excerpt from Vibrio parahaemolyticus (General): DBMD)


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